Why all the reads were classified as "no hits"

User Questions
1

Hello!

I want to inquire some problems during functional analysis on windows OS. I do import the results of blastx and load the GI mapping file for KEGG and SEED analysis setting (“gi2kegg-Feb2015X.bin” and “gi2seed.map”), but all my import reads can not be assigned. All the reads are classified as “no hits”.

Thank you for your time!

2

Hello which target database did you use ? in which format did you have your blastx files ?

Alessandro

3

I used the NCBI nr database and the outform 0, 5 of NCBI+

4

Was that XML then ? which version of Blast ? I can try and replicate here

Alessandro

5

Version: blast-2.3.0+ for linux system
I tried the 0 pairwise outfmt and 5 XML outfmt

6

Try again using diamond blastx with output in xml format

Alessandro