The MEGAN 6 paper is now online!

Rewati27 · June 22, 2016, 1:37pm

Here is the link :

ncbi.nlm.nih.gov

MEGAN Community Edition - Interactive Exploration and Analysis of Large-Scale Microbiome Sequencing Data.

DH Huson, S Beier, I Flade, A Górska, M El-Hadidi, S Mitra, HJ Ruscheweyh and R Tappu, PLoS computational biology, 2016 06

There is increasing interest in employing shotgun sequencing, rather than amplicon sequencing, to analyze microbiome samples. Typical projects may involve hundreds of samples and billions of sequencing reads. The comparison of such samples against a protein reference database generates billions of alignments and the analysis of such data is computationally challenging. To address this, we have substantially rewritten and extended our widely-used microbiome analysis tool MEGAN so as to facilitate the interactive analysis of the taxonomic and functional content of very large microbiome datasets. Other new features include a functional classifier called InterPro2GO, gene-centric read assembly, principal coordinate analysis of taxonomy and function, and support for metadata. The new program is called MEGAN Community Edition (CE) and is open source. By integrating MEGAN CE with our high-throughput DNA-to-protein alignment tool DIAMOND and by providing a new program MeganServer that allows access to metagenome analysis files hosted on a server, we provide a straightforward, yet powerful and complete pipeline for the analysis of metagenome shotgun sequences. We illustrate how to perform a full-scale computational analysis of a metagenomic sequencing project, involving 12 samples and 800 million reads, in less than three days on a single server. All source code is available here: https://github.com/danielhuson/megan-ce.

nerdynella · September 29, 2016, 10:46pm

For some reason I wasn’t able to access MEGAN community so I posted my question to the comments section of the journal article. Please see below:

Thank you for developing these user friendly tools for analyzing high throughput metagenomic seq data.
Could you please provide the parameter settings used to achieve these analyses runtimes? I am currently using your programs to analyze long reads (230bp) HiSeq metagenomic data and I am stuck at the DIAMOND stage. It would be very useful to see what settings were used and what trade-offs were made.
Thanks,
Nsa