Hello All,
I am trying to import BLAST tabular output into MEGAN with no great success.
This is the file: https://www.dropbox.com/s/w141xel83e3lzij/new-blast.txt?dl=0
I am not sure if I got the format correct as I could not locate it in the manual. Thank you.
This is what MEGAN expects in blast-tab format:
| Column | Data | Description |
|---|---|---|
| 1. | queryId | read id |
| 2. | subjectId | reference sequence id |
| 3. | pident | percent identity |
| 4. | length | alignment length |
| 5. | mismatch | number of mismatches |
| 6. | gapopen | number of gap openings |
| 7. | qstart | start of alignment in query |
| 8. | qend | end of alignment in query |
| 9. | sstart | start of alignment in subject |
| 10. | send | end of alignment in subject |
| 11. | evalue | expect value |
| 12. | bitscore | bit score |
Import of your data fails because your third value is much larger than 100 (so can’t be a percent identity)
Yes, all 12 columns must be present in the described order.
Hello, Daniel,
Could you please help answer Ziyi and my questions about MEGAN6.15.2 on Linux won’t open (MEGAN6.15.2 on Linux won't open)?
Thank you in advance!
Lily