Problem with multi-fasta reference for parasitic-worms

User Questions
1

I created a multi-fasta genome reference (of parasitic-worms). The header does not state any taxonomic classification.

grafik

The MALT-build output looked fine.

When I run a malt build + screen my samples this is the megan output:
grafik

My best guess is, that there is a step I did not take. I would have thought that the reference index with the megan-nucl-Feb2022.db.zip would be able to sort the taxonomy and the header of the concatenated genomic sequences are not important.
I anyone could give me a push in the right direction? I would be very grateful.

2

Looking at your header line, I don’t see any NCBI accession. That is what Malt would use to figure out what the taxon is. Can you put taxon ids into the header lines?