Dear Daniel,
To import peptides to MEGAN, I generate a “fake” blastp file like below:
BLASTP 2.9.0+
Query= AAAAAAGAGPEMVR//452437.6//1//P46196#P28482 length=14
·>P46196
Length=14
Score = 95.6 bits (218), Expect = 4e-21
Identities = 14/14 (100%), Positives = 14/14 (100%), Gaps = 0/14 (0%)
Query 1 AAAAAAGAGPEMVR 14
AAAAAAGAGPEMVR
Sbjct 1 AAAAAAGAGPEMVR 14
I use “import from blast” function to import the “blastp” result into MEGAN. The import is successful. I get Interpro and COG annotation.
However, I get no SEED, KEGG and VFDB mappings. Why?
The blastp file is here: blastp.rar (1.3 MB)
The accession to taxa fileuniprot_sprot.fasta.559634.acc2taxids.rar (2.4 MB)
Would you please give me a help? Any advice is appreciated!
Feng