Hi everybody! I am now writing this post beacuse I am confused the results of the eggnog function assignment. fitst, I blastp my amino acid sequence into eggnog database by diamond, and then import the blast.m8 file into the megan6, seting the parameters required, but found no assignments. I want to ask if any question about my eggnog database in version 4.0,or can anyone told me about how to do the eggnog function assignment with megan6 software!
thanks in advance