Hey guys,
I didn’t find a related previous topic, so I’m sorry if this has been asked befor.
I’m trying to do meganize on whole metagenome .daa files, but it keeps giving me the following error:
Info: Command completed (124s): meganize daaFile='C:\Users\carol\OneDrive\Área de Trabalho\DOUTORADO\Genes_phn\Metagenoma_diamond\Coleta 2019\diamond\01SR_1…
Executing: open viewer=SEED;
Executing: zoom what=fit;
Executing: open viewer=EGGNOG;
Executing: zoom what=fit;
Executing: uncollapse nodes=subtree;
Executing: uncollapse nodes=subtree;
Executing: show chart drawer=BarChart data=‘Taxonomy’;
Executing: open viewer=GTDB;
Executing: zoom what=fit;
Executing: uncollapse nodes=subtree;
Executing: open viewer=EC;
Executing: zoom what=fit;
Executing: uncollapse nodes=subtree;
Executing: open viewer=INTERPRO2GO;
Executing: zoom what=fit;
Executing: openUE viewer=KEGG;
Executing: zoom what=fit;
Executing: uncollapse nodes=subtree;
Executing: show window=MeganizeDAA;
Executing: load mapFile=‘C:\Users\carol\Downloads\MEGAN\megan-map-Jul2020-2-ue.db’ mapType=MeganMapDB cName=EC;
Executing: use cViewer=EC state=true;
Executing: load mapFile=‘C:\Users\carol\Downloads\MEGAN\megan-map-Jul2020-2-ue.db’ mapType=MeganMapDB cName=EGGNOG;
Executing: use cViewer=EGGNOG state=true;
Executing: load mapFile=‘C:\Users\carol\Downloads\MEGAN\megan-map-Jul2020-2-ue.db’ mapType=MeganMapDB cName=GTDB;
Executing: use cViewer=GTDB state=true;
Executing: load mapFile=‘C:\Users\carol\Downloads\MEGAN\megan-map-Jul2020-2-ue.db’ mapType=MeganMapDB cName=INTERPRO2GO;
Executing: use cViewer=INTERPRO2GO state=true;
Executing: load mapFile=‘C:\Users\carol\Downloads\MEGAN\megan-map-Jul2020-2-ue.db’ mapType=MeganMapDB cName=KEGG;
Executing: use cViewer=KEGG state=true;
Executing: load mapFile=‘C:\Users\carol\Downloads\MEGAN\megan-map-Jul2020-2-ue.db’ mapType=MeganMapDB cName=SEED;
Executing: use cViewer=SEED state=true;
Executing: load mapFile=‘C:\Users\carol\Downloads\MEGAN\megan-map-Jul2020-2-ue.db’ mapType=MeganMapDB cName=Taxonomy;
Executing: use cViewer=Taxonomy state=true;
Executing: update;
Executing: meganize daaFile=‘C:\Users\carol\OneDrive\Área de Trabalho\DOUTORADO\Metagenoma_MEGAN\04SR_1.daa’ minScore=50.0 maxExpected=0.01 minPercentIdentity=0.0 topPercent=10.0 minSupportPercent=0.05 lcaAlgorithm=naive minComplexity=0.0 useIdentityFilter=false readAssignmentMode=readCount fNames=EC EGGNOG GTDB INTERPRO2GO KEGG SEED longReads=false paired=false;
Meganizing file: C:\Users\carol\OneDrive\Área de Trabalho\DOUTORADO\Metagenoma_MEGAN\04SR_1.daa
Annotating DAA file using FAST mode (accession database and first accession per line)
NullPointerException: null
Command usage: meganize daaFile= [,…] [minScore=] [maxExpected=] [minPercentIdentity=]
[topPercent=] [minSupportPercent=] [minSupport=] [lcaAlgorithm={naive|weighted|longReads}] [lcaCoveragePercent=] [minPercentReadToCover=]
[minComplexity=] [useIdentityFilter={false|true}]
[fNames={EC|EGGNOG|GTDB|INTERPRO2GO|KEGG|SEED…} [longReads={false|true}] [paired={false|true} [pairSuffixLength={number}]]
[contaminantsFile=] [description=]; - Meganize DAA File
IOException: null
Error: Execute failed: java.io.IOException
However, when I do it for specific genes .daa files, it works fine. Don’t know what to do here. Can anyone help me?
Thank you.