Hi,
I am using diamond 0.9.14 and megan CE 6.10.6 on the command line (Ubuntu 16.04). I did a blastx with diamond against the ncbi nr database creating a daa file. Now I would like to parse the daa file with megan and add annotation. With earlier versions of diamond and megan it was possible to run tools like blast2rma. At the moment MEGAN seems not to support DAA files produces by Diamond:
daa2info -i sample.daa
Version MEGAN Community Edition (version 6.10.6, built 20 Dec 2017)
Copyright (C) 2017 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY.
Caught:
java.io.IOException: DAA version requires later version of MEGAN.
at megan.daa.io.DAAParser.isMeganizedDAAFile(DAAParser.java:99)
at megan.tools.DAA2Info.run(DAA2Info.java:107)
at megan.tools.DAA2Info.main(DAA2Info.java:52)
The DAA file produced by Diamond seems to be valid:
diamond view --daa sample.daa | head
HISEQ:176:HTMMKBCXX:2:1101:1846:2148 WP_060508806.1 100.0 87 0 0 263 3 147 233 2.0e-44 187.2
HISEQ:176:HTMMKBCXX:2:1101:1846:2148 WP_007200096.1 98.9 87 1 0 263 3 147 233 4.5e-44 186.0
HISEQ:176:HTMMKBCXX:2:1101:1846:2148 WP_003519193.1 98.9 87 1 0 263 3 147 233 4.5e-44 186.0
Which version of Diamond I need to run to get DAA files supported by Megan?
Thanks.
Mattias