Dear Sir or Madam,
We met problem in MEGAN6 regarding KEGG maps.
The system background:
The computer system: OS X Version 10.9.3.
MEGAN version: 6.1.32beta
KEGG: latest version (map*.conf and map*.png files uploaded to class/resource/kegg)
What I did:
Import *.rma6 files. Then click the kegg viewer. However, no KEGG maps can show in MEGAN.
Here is a screenshot:
Hope to get your help soon.
Thanks and regards,
due to licensing restrictions on KEGG, MEGAN does not ship with KEGG pathways. (MEGAN5 contained pathways dating back to 2011, before KEGG licensing changed.)
However, if you have a KEGG ftp license, then the Ultimate Edition of MEGAN allows you to view KEGG pathways within MEGAN using files downloaded from KEGG. To achieve this, using your licensed access to the KEGG ftp site, In this case, using your licensed access to the KEGG ftp site, download the KEGG files to a local directory.
Assuming that MEGAN 6 Ultimate Edition is installed in ~/megan and you have downloaded KEGG to ~/kegg, to enable visualization of KEGG pathways in MEGAN6, do the following:
tar xzf ~/kegg/xml/kgml/metabolic/ko.tar.gz
tar xzf ~/kegg/xml/kgml/non-metabolic/ko.tar.gz
mv ko/* .
mv map/.png .
cp ~/kegg/xml/kgml+/.xml .
tar xzf ~/kegg/pathway/map.tar.gz
This will give MEGAN access to all required files.
Then restart MEGAN6 Ultimate Edition. If you did everything correctly, then MEGAN will report KEGG bundled detected - will be able to display KEGG pathways in the message window, and you will be able to view KEGG pathways in the KEGG Viewer.
I am currently updating the user manual to explain exactly how to fully update MEGAN’s representation of KEGG if you have a KEGG ftp license.
Thanks a lot Daniel. It works!
I was wondering if it is possible to update KEGG on the Community Edition as well, because we have a license for KEGG.
At present, only the Ultimate Edition supports KEGG. In fact, KEGG is bundled into the UE.
I have a MEGAN6 UE trial version. How do I make it show KEGG pathways? The option “Show KEGG pathways…” is not lit up. I am running the program on Mac OS Mojave.
Did you meganize your DAA files or import your Blast files using the UE mapping database? megan-map-May2020-ue.db.zip
This contains the mapping to KEGG.
The KEGG analyzer is only available for files that have been meganized or created using that file.
Yes, my colleague imported the blast files using the UE mapping database on their Linux system and created .rma6 files. On their system he can view the KEGG pathways. When I open a file on my system, I get the following messages:
Version MEGAN Ultimate Edition (version 6.19.1, built 8 Jun 2020)
Author(s) Daniel H. Huson
Copyright © 2020 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY.
Java version: 14.0.1
License valid until: 2020-07-12
Opening startup files
Loading ncbi.map: 2,249,459
Loading ncbi.tre: 2,249,463
Executing: open file=’/Users/Astrid/Desktop/Coldcrab/Astrid_MEGANfiles/trinity_king16_MC.Trinity.fasta_diamond_blastx_nr_eval.1e-9.rma6’;
Info: Opened file ‘trinity_king16_MC.Trinity.fasta_diamond_blastx_nr_eval.1e-9.rma6’ with 14,779 reads
Executing: openUE viewer=KEGG;
Loading kegg.map: 23,979
Loading kegg.tre: 53,636
Executing: zoom what=fit;
And here is a screenshot:
You have to uncollapse the hierarchy displayed on the right until you can see some of the pathway nodes on the right. Then you must select the one of interest on the right and then the Show KEGG Pathway context menu item will be enabled.
(Sorry, uncollapsing only on the left does not enable the menu item…)