Even if we load the newest, alternate taxonomy files (generated with taxdmp2tree tool) into Megan CE, the daa-meganizer CLI command still insists on the default, older taxonomy data. Also, meganizing via GUI with the updated NCBI tree works smoothly. Unfortunately the tricks used in the Create-accession-db don’t work this time
What could be the solution?
Thanks in advance!
History:
/home/ngs_lab/megan/tools/daa-meganizer -i /home/ngs_lab/temp/*.daa -lg -top 0.1 -sup 1 -lcp 51 -ram readCount -alg longReads -a2t /home/ngs_lab/DB/Megan/accessionmap202402-virus.map -t 32 -v
Meganizer - Prepares (‘meganizes’) a DIAMOND .daa file for use with MEGAN
Java version: 20.0.2; max memory: 193.4G
Loading ncbi.map: 2,396,736
Loading ncbi.tre: 2,396,740
Loading file: /home/ngs_lab/DB/Megan/accessionmap202402-virus.map
Meganizing: /home/ngs_lab/temp/UNSGM-DryLab-2024-02.daa
Meganizing init
Annotating DAA file using EXTENDED mode
Annotating references
10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (1.3s)
Writing
10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (0.0s)
Binning reads Initializing…
Initializing binning…
Using ‘Interval-Union-LCA’ algorithm (51.0 %) for binning: Taxonomy
Binning reads…
Binning reads Analyzing alignments
10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (0.9s)
Total reads: 1,220
With hits: 929
Alignments: 3,042
Assig. Taxonomy: 782
Binning reads Applying min-support & disabled filter to Taxonomy…
10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (0.3s)
Min-supp. changes: 0
Binning reads Writing classification tables
10% 20% 30% 40% 50% 60% 70% 80% 90% 100% (0.0s)
Binning reads Syncing
100% (0.0s)
Class. Taxonomy: 288