Hi Daniel,
Thank you for creating such an amazing tool! I’m new to taxonomy profiling with long-read metagenomes and also relatively new to MEGAN. I’ve gone through several discussions here, but I haven’t come across one that specifically addresses my question.
I’m wondering if it’s possible to create a custom database using GTDB-annotated MAGs. I also have some NCBI eukaryotic genomes that I’d like to include in the database. Could you advise me on how to create a mapping file in this case? Some taxa in GTDB don’t have corresponding NCBI taxIDs, but I know you’ve also included GTDB mapping files in the nt/nr mdb, so I believe there should be a solution.
Thank you in advance for your help!
Hongyu