Hello everyone! How to perform taxonomic analysis by using Megan? Whether my analysis steps are right? See the file~~

help.doc (620.5 KB)

You need to provide alignments to MEGAN, not target sequences. See this tutorial for details: Tutorial · husonlab/tutorials Wiki · GitHub

Thanks! To begin the DIAMOND + MEGAN pipeline, the first step is to generate an index for the database file using DIAMOND. The DIAMOND has been installed by using brew (brew install diamond ) on my MacOS. However,for the Index generation, when typing “diamond makedb --in tutorial-nr.gz --db tutorial-nr” , the results will be :

. How to solve this problem?

did you download the data file and unzip it? Are you in the directory containing the file tutorial-nr.gz when you typed the command?

To verify, type

ls tutorial-nr.gz

If nothing is listed, the file is not present or you are in the wrong directory.

Please note that the tutorial does assume that you have some command-line knowledge. If you do not know how to create a directory, change directory, list files etc in Linux then please first work through a tutorial that teaches that because unfortunately, our tutorial does not provide that support.