help.doc (620.5 KB)
You need to provide alignments to MEGAN, not target sequences. See this tutorial for details: Tutorial · husonlab/tutorials Wiki · GitHub
Thanks! To begin the DIAMOND + MEGAN pipeline, the first step is to generate an index for the database file using DIAMOND. The DIAMOND has been installed by using brew (brew install diamond ) on my MacOS. However，for the Index generation， when typing “diamond makedb --in tutorial-nr.gz --db tutorial-nr” , the results will be :
did you download the data file and unzip it? Are you in the directory containing the file tutorial-nr.gz when you typed the command?
To verify, type
If nothing is listed, the file is not present or you are in the wrong directory.
Please note that the tutorial does assume that you have some command-line knowledge. If you do not know how to create a directory, change directory, list files etc in Linux then please first work through a tutorial that teaches that because unfortunately, our tutorial does not provide that support.