Error using taxonomy2function with KEGG

User Questions
1

Hi,
I am using the MEGAN6 Community Edition and want to map KEGG Ids to Taxonomy with taxonomy2function. It works fine, when I am using EGGNOG instead, but with KEGG I am getting the folowing error:

taxonomy2function -i LuW1_nA_SB_chop.daa -o LuW1_nA_SB_chop_KEGG.txt.gz -a KEGG -b Taxonomy -bf path

Processing: First classification
Caught:
java.lang.NullPointerException: Cannot invoke “megan.data.IClassificationBlock.getKeySet()” because “firstClassificationBlock” is null
at megan/megan.tools.Taxonomy2Function.run(Taxonomy2Function.java:177)
at megan/megan.tools.Taxonomy2Function.main(Taxonomy2Function.java:55)

Is this, because I am using the MEGAN6 Community Edition and not the UE?

All the best,
Torsten

2

Yes, the way that KEGG is used in MEGAN requires a KEGG license, which comes with a UE license.

3

Thanks,
I have requested a 30 days trail licence, which I have now installed. Do I have to run DIAMOND and Meganizer again on my fastq files, or can I use the existing meganized files, I have created for the Community Edition?

1 Like
4

OK, I need to run Meganizer again with the UE database.