Error message with .daa file

User Questions
1

Hi,

one of my .daa files constantly causes an error message when trying to meganize it or import it via “Import from Blast”. The error code is:

readNullTerminatedBytes(): failed (EOF)

BTW: this is the only file I I got from aligning via Diamond on a Linux computer. All the other .daa files that are accepted without complaints come from Windows computers.
And the erroneous file works well with command-line meganization via daa2rma.

Any chance to repair the defective file or do I have to run the Diamond alignment again?

Thank you,

Ralf

2

can you run daa2info on the file without problems?

3

Hi Daniel,

yes, it seems so. At least I get similar outputs with my problem file and another file that worked fine.

Like so (problem file):

#./daa2info -i NG-11226_269_lib157502_5050_7_1.daa [ 2:17]
Version MEGAN Community Edition (version 6.7.4, built 13 Mar 2017)
Copyright © 2017 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY.
Loading MEGAN File: NG-11226_269_lib157502_5050_7_1.daa
Caught:
java.io.IOException: DAA file has not been meganized: NG-11226_269_lib157502_5050_7_1.daa
at megan.daa.connector.DAAConnector.(DAAConnector.java:57)
at megan.core.MeganFile.getConnector(MeganFile.java:260)
at megan.core.Document.getConnector(Document.java:1008)
at megan.core.Document.loadMeganFile(Document.java:193)
at megan.tools.DAA2Info.run(DAA2Info.java:95)
at megan.tools.DAA2Info.main(DAA2Info.java:52)
Caught:
java.lang.NullPointerException
at megan.core.SyncArchiveAndDataTable.syncArchive2Summary(SyncArchiveAndDataTable.java:79)
at megan.core.Document.loadMeganFile(Document.java:194)
at megan.tools.DAA2Info.run(DAA2Info.java:95)
at megan.tools.DAA2Info.main(DAA2Info.java:52)

Thank you

Ralf

4

Dear Ralf,

Probably easiest if you rerun DIAMOND.
Before that, could you please see whether you can use DIAMOND and its view command to create e.g. a tab file.
If that fails, than that would suggest that the DAA file is corrupted, if that succeeds then it could be a bug in daa-meganzier, which would like to look into, if you can give me access to the file.
Thanks
daniel

5

Dear Daniel,

just tried DIAMOND view with my DAA file and got an Error: Error reading file…

So no need to do any debugging :wink:

Thank you,

Ralf