Daa2rma stopped at 70%

User Questions
1

Hi there,

I am using daa2rma program to convert two paired-end files with each 9062879 reads to single rma file but unfortunately, the program stopped at 70% without completion.

Version MEGAN Community Edition
Copyright © 2019 Daniel H. Huson. This program comes with ABSOLUTELY NO WARRANTY.
Functional classifications to use: EGGNOG, INTERPRO2GO, SEED
Loading ncbi.map: 2,175,506
Loading ncbi.tre: 2,175,510
Loading eggnog.map: 30,875
Loading eggnog.tre: 30,986
Loading interpro2go.map: 13,501
Loading interpro2go.tre: 29,204
Loading seed.map: 13,662
Loading seed.tre: 21,085
In DAA files: CDC016.R1.daa, CDC016.R2.daa
Output file: CDC016.rma
Classifications: Taxonomy, SEED, EGGNOG, INTERPRO2GO
Annotating RMA6 file using FAST mode (accession database and first accession per line)
Parsing file: CDC016.R1.daa
10% 20% 30% 40% 50% 60% 70% 80% 90% Parsing file: CDC016.R2.daa
10% 20% 30% 40% 50% 60% 70%

I wonder if this is something to do with the memory or other factors? How can I fix this?

Many thanks

2

How much memory did you give MEGAN? This appears at the bottom right of the MEGAN main window. Should be 8 G or preferably more. If on MacOS or Linux, edit the megan.vmoptions file in the installation directory to give the program more memory, if necessary.
Please let me know with how much memory you tried running the program. It might be that the new accession database code is using a lot of memory, in which case I will work on fixing that.