Daa-meganize all sequences not assigned

User Questions
1

Dear Megan team!
I run daa-meganizer using the line: $/Applications/MEGAN/tools/daa-meganizer -i /diamond_Out/49S.daa -fun EGGNOG INTERPRO2GO KEGG SEED -g2t /Applications/MEGAN/nucl-gi2taxid-August2016.bin -g2interpro2go /Applications/MEGAN/gi2interpro-June2016.bin -g2kegg /Applications/MEGAN/gi2kegg-Aug2016X-ue.bin -g2seed /Applications/MEGAN/gi2seed-May2015X.bin

However, when I upload in Megan6 all the sequences were not assigned, what i did wrong?

thank you so much!
best,

2

what database did you diamond-blast against?

3

Hi Daniel, I used nr from NCBI -

4

Here is the problem, you are using the wrong taxonomy mapping file:

This is for alignments against nucleotide sequences (hence the prefix “nucl”), please use
“prot_gi2tax-Aug2016X.bin” available from:
http://ab.inf.uni-tuebingen.de/data/software/megan6/download/prot_gi2tax-Aug2016X.bin.zip

1 Like
5

I`ve already tried with prot_gi2tax-Aug2016X.bin, but still the same! all sequences not assigned.

6

/Applications/MEGAN/tools/daa2rma -i Documents/projeto/Diamond_Out/diamond_Out/49S.daa -o Documents/projeto/Diamond_Out/diamond_Out/daa2rma6/49Staxprot.rma -fun EGGNOG INTERPRO2GO KEGG SEED -g2t /Applications/MEGAN/prot-gi2tax-August2016X.bin -g2interpro2go /Applications/MEGAN/gi2interpro-June2016.bin -g2kegg /Applications/MEGAN/gi2kegg-Aug2016X-ue.bin -g2seed /Applications/MEGAN/gi2seed-May2015X.bin

7

/Applications/MEGAN/tools/daa-meganizer -i Documents/projeto/Diamond_Out/diamond_Out/49S.daa -fun EGGNOG INTERPRO2GO KEGG SEED -g2t /Applications/MEGAN/prot-gi2tax-August2016X.bin -g2interpro2go /Applications/MEGAN/gi2interpro-June2016.bin -g2kegg /Applications/MEGAN/gi2kegg-Aug2016X-ue.bin -g2seed /Applications/MEGAN/gi2seed-May2015X.bin