Controlling false positive identifications

Hi Daniel.

Is it possible to create a decoy database out of the NCBI nr database.

Create a diamond index file out of this decoy database. Run a search and then Meganize the output to see the false identifications.

Please let me know if this is possible.

Hi @StevenKG,

Do you want to perform this on the entire NCBI-nr database? Please note that it could pose significant scalability challenges, as the current NCBI-nr contains around 812 million accessions. Or are you considering working with just a subset of the database (to test)?

Best regards,
Anupam